EFEITO DA INGESTÃO AGUDA DE CAFEÍNA NA RESPOSTA GLICÊMICA E INSULÍNICA EM RATOS DIABÉTICOS

Abstract

The diabetes mellitus associated with a group of diseases related to glucose metabolism, increasingly affecting the population, being a serious public health problem. The exercise increases the muscle glucose uptake during and post-effort. Studies have shown that the consumption of caffeine in the diet increases the Ca2+ concentration in pancreatic β cell, which is involved with insulin secretion in diabetic rats. However, little is known about these hormonal and metabolic adaptations associated with the acutely use of caffeine in association with exercise. The aim of this study is to investigate the effects of supplementation with caffeine associated with physical exercise on glucose and insulin response in diabetic rats. Methods: It was used 48 rats, with 60 days old and 116 ± 3g of weight. The induction of diabetes was conducted by administering 60 mg/kg of streptozotocin (STZ). Acutely, the animals received 6 mg/kg of caffeine, or 10 mg/kg sulfonylurea and saline for control groups. The rats remained fasting 6 hours. After supplement intake, there were 50 minutes of rest until the beginning of the exercise. Blood glucose was measured for capillary puncture by a portable glucometer, in fasting, pre-exercise and after exercise. At the end of exercise, the animals were sacrificed for blood collection and biochemical analyzes (blood glucose, glycerol, lactateand insulin) by colorimetric techniques using biochemical kits. Before and after the prescribing treatment, there was a control of cardiovascular responses using a tail plethysmograph. Results and Discussion: Prescription of caffeine in the dose of 6 mg/kg did not alter cardiovascular responses. However, caffeine promoted a significant reduction in blood glucose (42%) after 60 minutes of exercise protocol in diabetic rats compared to control groups. Insulin levels increased 127% to acute treatment with caffeine associated with sulfonylurea compared to other diabetic groups. Conclusion: In conclusion, the results show that acute ingestion of caffeine along with exercise can increase glucose uptake in peripheral consumption in skeletal muscle, and its association with drug sulfonylurea increase the release of insulin by the pancreas deficient in β-cells.