http://tede.unicentro.br:8080/jspui/handle/jspui/1825 Programa de Pós-Graduação em Nanociências e Biociências (Mestrado) 2026-06-06T12:05:13Z http://tede.unicentro.br:8080/jspui/handle/jspui/2036 Título: PRODUÇÃO E CARACTERIZAÇÃO DE SCAFFOLDS ELETROFIADOS A PARTIR DE POLICAPROLACTONA/PLURONIC/HIDROXIAPATITA PARA APLICAÇÕES BIOMÉDICAS Autor: RIBEIRO, LARISSA GULOGURSKI Primeiro orientador: Bonadio, Taiana Gabriela Moretti Abstract: Tissue engineering is currently being widely explored in various application branches. Among them, its use in medicine stands out regenerative, in a broad and versatile way, such as, for example, in the manufacture of micro and nanostructured bioactive materials, capazes de mimetizar a matriz extracelular e atuarem como curativos inteligentes em feridas crônicas. One of ways in which these materials are obtained is electrospinning, which allows the obtaining fibers in nanometric and micrometric scale, from a solution containing a volatile solvent and a polymer subjected to high stress. One of the polymers most used in the manufacture of these materials, and already approved by agencies of health, is polycaprolactone (PCL) because it is biodegradable and biocompatible. One problem in its characteristics is the hydrophobicity it presents, which can compromising the adhesion and proliferation of cells to the fibers. Such inconvenience can be resolved, for example, through the manufacture of polymeric blends and/or with the composite production. In this work, composites were produced from the blend polymeric PCL/F-127 with hydroxyapatite (HA) ceramic with a view to obtaining materials with bioactive properties to act as scaffolds in regeneration of living tissues in wounds. The PCL/F-127/HA composite was produced by the technique of electrospinning and characterized by different techniques: infrared spectroscopy, differential scanning calorimetry, thermogravimetry, electron microscopy by scanning and energy dispersion X-ray spectroscopy. The results showed that it was possible to obtain nano and micrometric fibers both from pure PCL, how much of the PCL blend with F-127 (PF) and the PF blend composite with particles of hydroxyapatite. Changes were noted in the polymeric chain and crystallinity of PCL both because of the electrospinning process and because of the addition of F-127. However, with the addition of hydroxyapatite there was a tendency to increase the crystallinity because it decreases the rate of evaporation of the solvent during the process of electrospinning. Tests performed on cultured human fibroblasts revealed that electrotrophied membranes have low cytotoxicity. Therefore, the materials developed in this work have the potential to be used in the future in biomedical applications. Instituição: Universidade Estadual do Centro-Oeste Tipo do documento: Dissertação 2022-03-04T00:00:00Z http://tede.unicentro.br:8080/jspui/handle/jspui/1846 Título: DAOXI – DISPOSITIVO PARA AUTOMAÇÃO DA OXIGENOTERAPIA Autor: RICKLI, CAMILA Primeiro orientador: Freitas, Valdirlei Fernandes Abstract: The present study is related to the development of a device that was created to carry out the automated control of the oxygen flow used by individuals who need oxygen suport. Oxygen therapy is used to improve blood oxygenation in patients who have some need for ventilatory support, by offering inhaled oxygen. The device attaches to manual flowmeters found in clinical/hospital settings to control parameters related to oxygen therapy. The Oxygen Therapy Automation Device - DAOXI controls oxygenation through external programming for reading an oximeter and mechanical actuation of the flowmeter. In this prototype, developed using the Internet of Things (IoT), we have a utility model for the automated control of oxygen flow, to control parameters related to oxygen therapy. DAOXI, through a software, performs the automated control and monitoring of the individual's blood oxygenation, making adjustments in the oxygen flow administered according to the desired Target Saturation for each patient. Through a data control and processing center, the device also promotes integration with a platform for recording, managing and analyzing data that allows issuing alerts, generating reports and using specific algorithms for intelligent control. Automated control will then be carried out by monitoring the individual's blood oxygenation and increasing or decreasing the flow of oxygen administered according to the required Target Saturation to be reached for each patient. Through an on-board data processing and control center, the device also has integration with a data recording and analysis platform that allows issuing alerts, generating reports, predicting oxygenation patterns intelligently and using specific algorithms for control. Bench tests were performed with the submission of the device in tests to evaluate the functioning of the algorithm, predicting different configured target saturations, observing the valve actuation and also, maintaining as visual flow feedback, observed in the analog ruler during the tests, comparing with the flow recorded on the device and system as part of the Product Validation process. Instituição: Universidade Estadual do Centro-Oeste Tipo do documento: Dissertação 2021-10-11T00:00:00Z http://tede.unicentro.br:8080/jspui/handle/jspui/1832 Título: ANÁLISE QUANTITATIVA E QUALITATIVA POR NANOPARTÍCULAS DE RESVERATROL EM MODELO DE NEUROINFLAMAÇÃO Autor: Lima, Mariane Maria Silveira Vieira de Primeiro orientador: Kerppers, Ivo Ilvan Abstract: Over the past few years, AD has been studied as a denomination of neurodegenerative dysfunction, a disease that acts progressively with the accumulation of Beta Amyloid (Aβ). Although the exact cause of AD needs to be further elucidated due to its complexity, growing lines highlight that the accumulation of Aβ protein aggregates is one of the causative events in the pathogenesis of AD. The accumulation of these protein aggregates triggers neuroinflammation, oxidative stress and mitochondrial damage, leading to the loss of neurons. Thus, a great promise is Resveratrol (anti-inflammatory, antioxidant, antitumor and neuroprotective). Therefore, this study aims to evaluate the effects of zein nanoparticles containing resveratrol in an experimental model of neuroinflammation. This is an experimental, descriptive and observational study being carried out in the Neuroanatomy laboratory of the State University of the Center West (UNICENTRO ). Sample consisted of 30 animals divided into control (C), positive control (CP), white nanoparticles (NB), Resveratrol nanoparticles (NR) and resveratrol (R). The animals received 10mg/kg daily, diluted in 0, 5 ml for 15 days. Afterwards, they were submitted to surgery and kept for 30 days for immunohistochemical (IHC) analysis. The results showed that in the qualitative analysis of the IHC, to assess the deposition of Anti Beta Amyloid, there was no change in its morphological brain composition in the NR and C groups, unlike the CP, NB and R groups, which showed changes in the deposition of Anti Tau. it appears that in the NR group there was a normal projection of taurine in the axon which did not correspond in the same way to the other groups, for the CD68 marker in the R and C groups there was no microglial activation. Quantitative analyzes of Anti Beta Amyloid in the NR group showed a statistical difference when compared to the CP, NB and R groups, whereas in the Anti Tau analysis there was a significant difference between the CP and NR groups (p=0.0001), and by end for marker CD68 there was a significant result (p=0.0014) for C and NR. In the analysis of pro and anti-inflammatory cytokines such as IL-10, IL-6, TNF-α and InF-δ that were evaluated by flow cytometry and showing a significant difference in TNF-α between groups C, CP being (p=0.0272) , between group C and NB with the difference of (p=0.0076), CP and NR (p=0.0240) and NB and NR with (p=0.0065), the only group with no statistical difference was the R group. In the analysis of IL-6 and InF -δ showed no significant difference. In IL-10, however, it showed significance between groups C and NR with (p=0.001), group C and R with (p= 0.022) and finally the group CP and NR with a statistical difference being (p=0.0169). It is concluded that NR prevented the evolution of neuroinflammation caused by the induction of beta amyloid1-42 in the hippocampal region CA1. Instituição: Universidade Estadual do Centro-Oeste Tipo do documento: Dissertação 2021-08-16T00:00:00Z http://tede.unicentro.br:8080/jspui/handle/jspui/1831 Título: DESENVOLVIMENTO DE CURATIVO MULTIFUNCIONAL PARA TRATAMENTO DE FERIDAS CRÔNICAS POR TERAPIA FOTODINÂMICA Autor: Grossko, Douglas Primeiro orientador: Tominaga, Tania Toyomi Abstract: The chronic wounds healing it is a complex task and a public health issue. Therefore, the development of efficient bandages it is extremely important to the treatment of this kind of injury. Bandages that are made of electrospinning fibers have a three-dimensional porous structure that give the capability of absorbing exudates, diffusing vapours and allowing for cell proliferation by the material. The curcumin (CUR) incorporation as a photosensitizer to the photodynamic therapy (PDT) turns the electrospinning fibers a multifunctional material able to act in the healing process fighting infections and stimulating cell regeneration. Thus, the essence of this paper is in development of nanostructured materials through the electrospinning technique, incorporating the CUR to the PDT wound treatment. The physico-chemical study of photosensitizer has revealed that the CUR presents spectroscopic properties suitable to the treatment of superficial wounds. The fiber was electrospinning from polymer blends of polycaprolactone (PCL) and Pluronic® F-108. The scanning electron microscopy (SEM) showed the fiber formation of average diameters between 616 nm and 1134 nm. The release testing demonstrated a fast release rate that it is affected by the F-108 concentration. The results indicate a promising material to be used in the PDT wound treatment. Instituição: Universidade Estadual do Centro-Oeste Tipo do documento: Dissertação 2021-10-01T00:00:00Z