ANÁLISE DE EXPRESSÃO GÊNICA DE DUODENO E RIM DE GALINHAS POEDEIRAS SUBMETIDAS A DIETAS COM DIFERENTES NÍVEIS DE CÁLCIO E FÓSFORO

Abstract

The minerals calcium (Ca) and phosphorus (P) are essential in the diet of laying hens, participating in many metabolic functions, in addition in the diet of laying hens, participating in many metabolic functions, in addition to eggshell formation. However, the different levels can result in a big difference, due to the reduction of the mineral’s absorption capacity. Therefore, the objetictive of this work was to profile the expression of Ca and P-related genes, using duodenum and kidney of older laying hens. Samples were collected from Bovans White laying hens, aged 73 weeks, fron three groups whith different levels of performance and Ca/P retio in the diet, as follows: (HP) high performance (22.43 Ca/P), low performance (HL) 6.71% Ca/P and the standart diet (STD) commomly used 11.43 Ca/P. For the study, RNA sequencing was performed with 27 samples from the duodenum (9/group), using the RNA-Seq technique. For kidney expression, 30 kidney samples (10/group) were selected, evaluation analysis of the chosen genes, being: CYP24A1, FG3R, PTH, TRPV6, CALB1, CALM2, FGF1 and TRPC3. As a result, considering the comparisons between groups, 107 genes were enriched in mineral homeostasis processes, highlighting the genes: CAV1, MYLK, CAB39L, ILIRLI, TNIP3, COL3A1, PKIA, FGF7, ESRRG, PTHLH, ADIPOQ e ESRRG. No rim, os genes CYP24A1 and TRCP3 genes were DE between HP and STD groups and between lp and std, respectively. In this study, we identified new candidate genes for calcium and phosphorus mechanisms in older laying hens.