DESENVOLVIMENTO E CARACTERIZAÇÃO DE NANOPARTÍCULAS DE ALBUMINA CONTENDO MELATON

Abstract

Nanotechnology is involved in the manipulation of atoms and molecules, and its concept is the creation of new nanomaterials and devices. Within the pharmaceutical industry, its use for pharmacological application has grown in order to circumvent pharmacodynamic and pharmacokinetic problems such as low efficacy or lack of selectivity as well as the intrinsic toxicity of various compounds. Albumin is a protein abundant in the organic system, accounting for about 50% of the total surrounding protein. Its ability to carry insoluble molecules gives it a property of, binding itself to different molecules and ions. Albumin-based nanoparticles are shown as an option, since a significant number of drugs can be incorporated into their matrix. Melatonin (MEL), a hormone secreted by the mammalian pineal gland, has been shown in recent research to be more effective than other known antioxidants. It is an excellent free radical scavenger, the greatest impasse in its use and its rapid organic metabolism. The objective of this work was to develop BSA NPs containing MEL, as well as to determine their morphological, physical-chemical characteristics and to evaluate the profile on normal cells. The average diameter of melatonin-containing albumin nanoparticles was 205nm (± 2.2nm) and mean polydispersity index mean was 0.085 (± 0.02), mean encapsulation efficiency was 70.5% (± 3, 5%). All nanoparticles remained stable for up to 60 days, both at room temperature and refrigerated. They have spherical shape and smooth surface and in their physical-chemical characteristics we have an amorphous profile, desirable characteristics for an efficient nanostructured, drug carrier system. In cytotoxic assays MEL does not alter the growth of Vero cells, nor does it cause hemolysis of the red blood cells. Considering the low toxicity of this system, it can be used as a platform for oral and staggered applications.